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KMID : 0358119830090020061
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Journal of the Korean Public Health Association
1983 Volume.9 No. 2 p.61 ~ p.66
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A STUDY ON THE PEPTIDE DISTRIBUTION CHARACTERISTICS OF MRS CULTURE OF LACTOBACILLUS spp AND THEIR INHIBITORY ACTIVITIES AGAINST ESCHERICHIA coil.
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Abstract
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A study on the peptide distribution characteristics of MRS culture of Lactobacillus -spp and their
-inhibitory activity against Escherichia coli was conducted by high performance liquid chromatography and cylinder plate assay method. The following results were obtained;
1. Nine peptides were isolated in MRS broth and their molecular weight ranged from. 38, 000 daltons to 3, 800 daltons. The contents of peptides showed that the peptide of 38, 000 dalton in molecular weight was most abundant with 30.9 percent of total peptides.
2. Changes in peptide content occurred during the cultivation period of 120 hours, at 37°C after inoculation with. Lactobacillus spp. Peptides of 35, 000 dalton and 21, 000 dalton were consumed in a large scale and ´ the peptide profiles of MRS broth were so specific depending upon the species or strains of the organisms that they can be used as a reference for differentiation of species or "strains of Lactobacillus spp.
2. A general inhibitory activity of Lactobacillus spp against Escherichia coli with a particular intensive effect upon pathogenic strains and a great reduction in this effect by heat and alkali treatments have been observed. There was no definite interrelationships between intensity of inhibitory activity and peptide profile of Lactobacilli spp MRS culture.
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KEYWORD
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